Effects of ATP on expression of inflammatory factors in endothelial progenitor cells induced by LPS and the mechanisms
1. Department of Geriatric Cardiology; National Center for Clinical Research of Geriatric Diseases, Xiangya Hospital, Central South University, Changsha 410008; 2. School of Stomatology, Lanzhou University, Lanzhou 710000, China
Abstract Objective: To investigate the effects of adenosine triphosphate (ATP) on expression of
infl ammatory factors induced by lipopolysaccharide (LPS) in endothelial progenitor cells (EPCs),and to elucidate the possible mechanisms.
Methods: Mononuclear cells were isolated from human umbilical cord blood by density gradient
centrifugation, RT-PCR was performed to detect the expression of inflammatory factors induced
by LPS (1 mg/mL) in EPCs, the effect of low concentration (5 μmol/L) of ATP on expression of
IL-1β, MCP-1 and ICAM-1, and the effect of different concentrations (5, 50 μmol/L) of ATP on
the expression of Toll-like receptor (TLR) 4, myeloid differentiation primary response protein 88
(MyD88) and CD14. Western blot was performed to detect expression of TLR4 regulated proteins
MyD88 and CD14 or to detect the low concentration (1, 5 μmol/L) of ATP on the expression of
TLR4, MyD88 and CD14 and the NF-κB signaling pathway.
Results: EPCs highly expressed TLR4, and its ligand LPS (1 mg/mL) significantly upregulated
mRNA expression of IL-1β, MCP-1 and ICAM-1 and protein expression of MyD88 and CD14 in a
time-dependent manner (P<0.01), accompanied by activation of ERK and NF-κB signal pathway.
ATP at low concentration (5 μmol/L) significantly inhibited LPS-induced mRNA expression of
IL-1β, MCP-1 and ICAM-1(P<0.05), downregulated the LPS-induced protein expression of TLR4,
MyD88 and CD14 in EPCs (P<0.05), and suppressed LPS-induced activation of NF-κB signaling
Conclusion: ATP at low concentration may suppress LPS-induced expression of inflammatory
factors in EPCs through negative regulation of the TLR4 signaling pathway.
XIAO Bolin1,2,CHEN Meifang1等. Effects of ATP on expression of inflammatory factors in endothelial progenitor cells induced by LPS and the mechanisms[J]. Journal of Central South University(Medical Scienc, 2018, 43(12): 1301-1308.