中南大学学报(医学版)
      首页 | 期刊介绍 | 编委会 | 投稿指南 | 期刊订阅 | 广告合作 | 编辑部公告 | 下载中心 | 留言板 | 期刊下载 | 联系我们 | English
J4  2014, Vol. 39 Issue (5): 477-482    DOI: 10.11817/j.issn.1672-7347.2014.05.007
论著 最新目录| 下期目录| 过刊浏览| 高级检索 |
应激对缺血预适应心肌细胞凋亡的影响
目的:研究应激反应对缺血预适应心肌细胞凋亡的影响。方法:将24只大白兔随机分4组:静脉用依托咪
酯建立兔去应激动物模型作为去应激预适应组(Etom)、正常应激预适应组(IPC)、甲基强的松龙组(MP)和无预适应
处理组(IR),用DNA-ladder、TUNEL 染色和Hoechst染色对比4组行缺血处理后细胞凋亡程度。结果:DNA-Ladder显
示条带密度IR组>Etom组>MP组>IPC组。TUNEL染色检测的凋亡率:Etom组(3.0%±0.4%)和IR组(3.8%±1.3%)与IPC组
(1.7%±0.2%)比较,差异有统计学意义(P<0.05);IR组和MP组(2.3%±0.8%)比较,差异有统计学意义(P<0.05)。Hoechst染
色检测的凋亡率:Etom组(7.6%±0.4%)和IR组(6.2%±1.6%)与IPC组(4.1%±0.9%)比较,差异有统计学意义(P<0.05);IR组
和MP组(3.5%±0.4%)比较,差异有统计学意义(P<0.05)。结论:去应激削弱了预适应对心肌细胞凋亡的抑制作用,甲
基强的松龙可抑制缺血再灌注损伤的心肌细胞凋亡。
1. 郑州大学第一附属医院心血管外科,郑州 450052;2. 中南大学湘雅医院心胸外科,长沙 410008
Effect of stress on myocardial apoptosis in ischemic preconditioning in rabbit hearts
Objective: To determine the influence of stress on myocardial apoptosis in ischemic
preconditioning group (IPC).
Methods: Twenty-four Japanese white rabbits were randomly divided into 4 groups (n=6): an
etomidate group (the Etom group) of depressed stress established by intravenous etomidate, an
IPC group, an ischemic reperfusion group (the IR group) and a methylprednisolone group (the MP
group). Myocardial apoptosis was examined by DNA-laddering, in situ nick-end labeling (TUNEL)
and Hoechst dyeing.
Results: The DNA ladder increased in the Etom group. The percentage of apoptosis by TUNEL
method was 1.7%±0.2% in the IPC group, 2.3%±0.8% in the MP group, 3.8%±1.3% in the IR group
and 3.0%±0.4% in the Etom group. Hoechst dying was 4.1%±0.9% in the IPC group, 3.5%±0.4% in the MP group, 6.2%±1.6% in the IR group and 7.6%±0.4% in the Etom group. There was significant
difference between the IPC group and the Etom group or IR group, and also between the MP group
and the IR group.
Conclusion: A depressed stress response impairs the inhibition on myocardial apoptosis in
ischemic preconditioning. Methylprednisolone may inhibit myocardial apoptosis.
1. Department of Cardiovascular Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052;
2. Department of Cardiothoracic Surgery, Xiangya Hospital, Central South University, Changsha 410008, China

版权所有 © 2009 《中南大学学报(医学版)》编辑部 ICP备案序号:湘ICP备09001153号
本系统由北京玛格泰克科技发展有限公司设计开发  技术支持:support@magtech.com.cn