[an error occurred while processing this directive] ���ϴ�ѧѧ��(ҽѧ��) 2013, 38(5) 483-489 DOI:   10.3969/j.issn.1672-7347.2013.05.007  ISSN: 1672-7347 CN: 43-1427/R

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Article by YANG Qiong
Article by YANG Kan
Article by LI Anying
Article by TAN Wenpeng
����1, ��٩1, �Ө1, ̷����2
1. ���ϴ�ѧ������ҽԺ���ڿ�, ��ɳ 410013;
2. ����ҽѧԺ��������ҽԺ���ڿ�, ���� 510150
ժҪ�� Ŀ��: �۲�microRNA-21(miR-21)��ȱѪ�ٹ�ע����(I/R)���ڴ����ļ��ı�P��ϸ��������Ӱ�졣����: SD ���������Ϊ������(��״����ע��תȾ�հ׶��ղ���rAAV9-ZsGreen),miR-21 ��(��״����ע��תȾrAAV9-ZsGreen-pre-miR-21),Sham ��(�����ع���),I/R ��(����I/R ����),I/R+miR-21(תȾrAAV9-ZsGreen-premiR-21 ���ٽ���I/R ����)����Realtime-PCR ������miR-21 ˮƽ,������֯��ѧ��Western ӡ���������Bcl-2,Bax,caspase-3 ˮƽ������Bcl-2/Bax ��ֵ�����: ��״����ע��rAAV9-ZsGreen-pre-miR-21 ��ʹmiR-21 �������4.43��(P<0.001);��Sham ��Ƚ�,I/R �����ȱѪ��miR-21 ˮƽ�µ�(P<0.05),����ȱѪ��miR-21 ����(P<0.05);��Sham ��Ƚ�,I/R ���ٹ�ע1,2,6 h miR-21 ˮƽ�������½�(P<0.05),��ͬһʱ���I/R+miR-21 ���I/R ��miR-21�������(P<0.05);��Sham��Ƚ�,�ٹ�ע6 h ʱI/R ��I/R+miR-21 ��Bcl-2 ���ߡ�caspase-3 ���ߡ�Bcl-2/Bax ����(P<0.05),��I/R ��Ƚ�,I/R+miR-21 ��Bcl-2 �µ���caspase-3 �µ���Bcl-2/Bax ����(P<0.05)������: I/R ���ڴ����ļ�ȱѪ��miR-21 ����½�,ϸ����������,�����miR-21 �ɸ���ϸ��������
�ؼ����� microRNA   microRNA-21   ȱѪ�ٹ�ע����   ϸ������  
Anti-apoptosis and expression of microRNA-21 in rat myocardium during early ischemia-reperfusion injury
YANG Qiong1, YANG Kan1, LI Anying1, TAN Wenpeng2
1. Department of Cardiology, Third Xiangya Hospital, Central South University, Changsha 410013;
2. Department of Cardiology, Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China
Abstract: Objective: To observe the expression and anti-apoptosis of microRNA-21(miR-21) in rat myocardium during early ischemia-reperfusion injury (I/R).
Methods: Sprague-Dawley rats were randomly divided into 5 groups: a control group (transfected with rAAV9-ZsGreen by coronary injection), a miR-21group (transfected rAAV9-ZsGreen-premiR- 21 by coronary injection), a sham group (open-chest only), an I/R group (I/R), and an I/ R+miR-21 (I/R after transfected rAAV9-ZsGreen-pre-miR-21 by coronary injection). Realtime PCR was used to assess the expression level of miR-21. Immunohistochemistry and Western blot were used to determine the expression of Bcl-2, Bax, caspase-3 and Bcl-2/Bax.
Results: MiR-21 was increased by 4.43 times in the miR-21 group (P<0.001). MiR-21 was downregulated in the ischemia zone after I/R compared with the sham group (P<0.05), but that in the non-ischemia zone was significantly increased compared with the sham group (P<0.01). MiR- 21 expression was decreased in the I/R group compared with that in the sham group at 1 h, 2 h and 6 h after I/R (P<0.05), and it was up-regulated in the I/R+miR-21 group at the same time point compared with the I/R group (P<0.01). The expression of Bcl-2, Bax, and caspase-3 was upregulated and Bcl-2/Bax was decreased in the ischemia zone in the I/R group and I/R+miR-21 group than the sham group(P<0.05). Compared with the I/R group, the expression of Bcl-2 and caspase-3 was down-regulated and Bcl-2/Bax was increased in the ischemia zone in the I/ R+miR-21 group (P<0.05).
Conclusion: MiR-21 expression is down-regulated and cell apoptosis is increased in rat myocardium during early ischemia-reperfusion injury. Myocardial cell apoptosis may be alleviated by miR-21 over-expression.
Keywords: microRNA   microRNA-21   ischemia-reperfusion injury   apoptosis  
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DOI: 10.3969/j.issn.1672-7347.2013.05.007

ͨѶ����: ��٩,Email: yangkan@medmail.com.cn
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����Email: yangkan@medmail.com.cn

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